This also means that, while she may still be friendly in The Fort Ikon Prison in a difficulty that you befriended her, you will no longer be able to buy her augments, in any difficulty.
Changing your mind with her in a new difficulty will cause your Outcast reputation to reset to 0 (Tolerated) in all difficulties. However, with Anasteria it is a little different. Talk to her and decide whether to kill her or let her live.